CD81 Antibody for Western Blot | Rabbit Polyclonal

	Catalog No	Formulation	Size	Price (USD)
	RQ4082	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

CD81 Antibody for Western Blot Human, Mouse and Rat. Western blot analysis of CD81/TAPA1 in human PC-3, Ramos, 293T and Jurkat cell lysates, rat PC-12 and C6 cell lysates, and mouse RAW264.7 and ANA-1 cell lysates using CD81 Antibody for Western Blot. Lane 1: PC-3; Lane 2: Ramos; Lane 3: 293T; Lane 4: Jurkat; Lane 5: rat PC-12; Lane 6: rat C6; Lane 7: mouse RAW264.7; Lane 8: mouse ANA-1. A specific band is detected at approximately 26 kDa, consistent with the expected molecular weight of CD81/TAPA1. This CD81 Antibody for Western Blot detects CD81 across human, rat and mouse samples and supports studies of TAPA-1 expression, tetraspanin biology and membrane-associated signaling.

CD81 Antibody Human Tonsil IHC. IHC staining of FFPE human tonsil tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4Â°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed in lymphoid cells within the tonsil. This CD81 Antibody demonstrates recognition of TAPA-1 in human lymphoid tissue and supports studies of CD81 expression, tetraspanin biology and membrane organization.

CD81 Antibody Human Tonsil Lymphoid Tissue IHC. IHC staining of FFPE human tonsil tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4Â°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed within the lymphoid tissue. This CD81 Antibody demonstrates recognition of TAPA-1 in human tonsil and supports studies of CD81 expression, immune cell biology and tetraspanin membrane organization.

CD81 Antibody Human Liver IHC. IHC staining of FFPE human liver tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4Â°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed in the liver tissue. This CD81 Antibody demonstrates recognition of TAPA-1 in human liver and supports studies of CD81 expression, tetraspanin biology and membrane organization.

CD81 Antibody Human Testis IHC. IHC staining of FFPE human testis tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4Â°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed in the testicular tissue. This CD81 Antibody demonstrates recognition of TAPA-1 in human testis and supports studies of CD81 expression, tetraspanin biology and membrane organization.

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CD81 Antibody for Western Blot Human, Mouse and Rat. Western blot analysis of CD81/TAPA1 in human PC-3, Ramos, 293T and Jurkat cell lysates, rat PC-12 and C6 cell lysates, and mouse RAW264.7 and ANA-1 cell lysates using CD81 Antibody for Western Blot. Lane 1: PC-3; Lane 2: Ramos; Lane 3: 293T; Lane 4: Jurkat; Lane 5: rat PC-12; Lane 6: rat C6; Lane 7: mouse RAW264.7; Lane 8: mouse ANA-1. A specific band is detected at approximately 26 kDa, consistent with the expected molecular weight of CD81/TAPA1. This CD81 Antibody for Western Blot detects CD81 across human, rat and mouse samples and supports studies of TAPA-1 expression, tetraspanin biology and membrane-associated signaling.

CD81 Antibody Human Tonsil IHC. IHC staining of FFPE human tonsil tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed in lymphoid cells within the tonsil. This CD81 Antibody demonstrates recognition of TAPA-1 in human lymphoid tissue and supports studies of CD81 expression, tetraspanin biology and membrane organization.

CD81 Antibody Human Tonsil Lymphoid Tissue IHC. IHC staining of FFPE human tonsil tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed within the lymphoid tissue. This CD81 Antibody demonstrates recognition of TAPA-1 in human tonsil and supports studies of CD81 expression, immune cell biology and tetraspanin membrane organization.

CD81 Antibody Human Liver IHC. IHC staining of FFPE human liver tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed in the liver tissue. This CD81 Antibody demonstrates recognition of TAPA-1 in human liver and supports studies of CD81 expression, tetraspanin biology and membrane organization.

CD81 Antibody Human Testis IHC. IHC staining of FFPE human testis tissue using CD81 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive CD81/TAPA1 staining is observed in the testicular tissue. This CD81 Antibody demonstrates recognition of TAPA-1 in human testis and supports studies of CD81 expression, tetraspanin biology and membrane organization.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P60033
Localization	Cytoplasmic, membranous
Applications	Immunohistochemistry (FFPE) : 2-5ug/ml Western Blot : 0.5-1ug/ml Direct ELISA : 0.1-0.5ug/ml
Limitations	This CD81 Antibody for Western Blot is available for research use only.
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Description

CD81 Antibody for Western Blot recognizes CD81, a widely expressed integral membrane protein and member of the tetraspanin family. Also known as TAPA-1 and Tetraspanin-28, CD81 contains four transmembrane domains and participates in the organization of protein complexes at the cell surface. Tetraspanins interact with receptors, integrins and other membrane-associated proteins to form specialized membrane domains that influence signaling and cellular interactions. Detection of CD81 by western blot can be useful for examining its expression in cell and tissue lysates and comparing relative protein abundance among experimental samples.

CD81 contributes to tetraspanin-enriched membrane domains that coordinate the localization and interactions of numerous cell-surface proteins. Through these molecular associations, CD81 participates in processes including cell adhesion, migration, proliferation and signal transduction. Rather than functioning primarily as an enzyme or conventional signaling receptor, CD81 acts as a membrane organizer whose biological effects depend on its interacting partners and cellular context. A CD81 Antibody for Western Blot can support biochemical analysis of this tetraspanin across different cell types and tissues.

CD81 has particularly important functions in immune cell biology. It is expressed on numerous leukocyte populations and associates with CD19 and CD21 as part of the B-cell coreceptor complex, contributing to regulation of B-cell signaling. CD81 has also been investigated in T-cell biology and other mechanisms involving immune cell activation and communication. These functions connect CD81 with the broader family of CD molecules used to characterize cell-surface phenotype and investigate mechanisms of immune signaling.

Beyond immune biology, CD81 is extensively studied in extracellular vesicles and pathogen-host interactions. CD81 is commonly associated with exosomes and other extracellular vesicle populations and is frequently examined together with the tetraspanins CD9 and CD63. It also functions as an important host factor in hepatitis C virus entry. These diverse roles have made CD81 relevant to studies of extracellular communication, membrane trafficking, infection and cancer biology. Western blot analysis provides a complementary approach for studying CD81 protein expression alongside methods that examine its cellular or membrane localization.

Because CD81 is a relatively small multipass membrane glycoprotein, its electrophoretic behavior can be influenced by sample preparation and experimental conditions. Evaluation of an antibody against multiple biological samples can therefore provide useful evidence for target recognition at the expected molecular weight. NSJ Bioreagents supplies antibodies to CD81 and other CD proteins involved in membrane organization and cell signaling. A CD81 Antibody for Western Blot can support studies of TAPA-1 expression, tetraspanin biology, immune signaling and extracellular vesicle research.

For additional information on CD81/TAPA-1 and its roles in tetraspanin membrane organization, immune signaling and cellular communication, see our recombinant CD81 Antibody page.

Application Notes

Optimal dilution of the CD81 Antibody for Western Blot should be determined by the researcher.

Immunogen

A recombinant human partial protein corresponding to amino acids F113-K201 was used as the immunogen for the CD81 Antibody.

Storage

After reconstitution, the CD81 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

CD81 antibody, CD81 Western Blot antibody, TAPA-1 antibody, TAPA1 antibody, Tetraspanin-28 antibody, TSPAN28 antibody, Target of Antiproliferative Antibody 1 antibody

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CD81 Antibody for Western Blot (RQ4082)

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NSJ Bioreagents