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Home >> Antibodies >> CD80 Antibody

CD80 Antibody (RQ4248)

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  Catalog No Formulation Size Price (USD)  
Image RQ4248 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC testing of FFPE human tonsil tissue with CD80 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human tonsil tissue with CD80 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat spleen tissue with CD80 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P33681
Localization Membrane
Applications IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This CD80 antibody is available for research use only.
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Description

Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.

Application Notes

Optimal dilution of the CD80 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids V35-L244 was used as the immunogen for the CD80 antibody.

Storage

After reconstitution, the CD80 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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