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Home >> Antibodies >> CD63 Antibody / LAMP-3

CD63 Antibody / LAMP-3 [clone rLAMP3/8604] (V4294)

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  Catalog No Formulation Size Price (USD)  
Image V4294-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4294-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4294SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with CD63 antibody (clone rLAMP3/8604). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with CD63 antibody (clone rLAMP3/8604). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD63 antibody (clone rLAMP3/8604) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rLAMP3/8604
Purity Protein A/G affinity
UniProt P08962
Localization Cell Surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD63 antibody is available for research use only.
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Description

This mAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of mAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Application Notes

Optimal dilution of the CD63 antibody should be determined by the researcher.

Immunogen

The smooth plasma membrane fraction of MeWo cells was used as the immunogen for the CD63 antibody.

Storage

Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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