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Home >> Antibodies >> CD63 Antibody PE Conjugate

CD63 Antibody PE Conjugate [clone MX-49.129.5] (V2071PE)

  Catalog No Formulation Size Price (USD)  
Image V2071PE-100T 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 Tests 519
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FACS testing of human PBMC: Black=cells alone; Green=isotype control; Red=CD63 antibody
FACS testing of mouse NIH3T3 cells: Black=cells alone; Green=isotype control; Red=CD63 antibody
Availability 1-3 business days
Species Reactivity Human and Mouse. Other species not known.
Format PE Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MX-49.129.5
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 967 (Human)
Localization Cytoplasmic
Applications Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood
Immunofluorescence : 1:50-1:100 for 30 minutes at RT
Limitations This CD63 antibody is available for research use only.
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Description

The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Immunogen

Full length human CD63 was used as the immunogen for this antibody.

Storage

Store the CD63 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

Alternate Names

gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30, CD63 antibody

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