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Home >> Antibodies >> CD63 Antibody / LAMP-3

CD63 Antibody / LAMP-3 (F49127)

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  Catalog No Formulation Size Price (USD)  
Image F49127-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
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F49127-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot testing of CD63 antibody at 1:2000 dilution. Lane 1: A2058 lysate; 2: A375 lysate; 3: THP-1 lysate; 4: U-87 MG lysate; Predicted molecular weight: 25-60 kDa depending on glycosylation level.
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Western blot testing of CD63 antibody at 1:2000 dilution. Lane 1: A2058 lysate; 2: A375 lysate; 3: THP-1 lysate; 4: U-87 MG lysate; Predicted molecular weight: 25-60 kDa depending on glycosylation level.
Western blot testing of CD63 antibody at 1:1000 dilution + human plasma lysate; Predicted molecular weight: 25-60 kDa depending on glycosylation level.
Western blot testing of CD63 antibody at 1:1000 dilution. Lane 1: A2058 lysate; 2: MDA-MB-453 lysate; Predicted molecular weight: 25-60 kDa depending on glycosylation level.
Western blot testing of human spleen tissue lysate with CD63 antibody. Predicted molecular weight: 25-60 kDa depending on glycosylation level.
FACS testing of fixed and permeabilized human HL-60 cells with CD63 antibody at 1:25 dilution. Blue = isotype control, Green = CD63 antibody.
IHC testing of FFPE human melanoma tissue with CD63 antibody at 1:25 dilution. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human skin with CD63 antibody at 1:25 dilution. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human tonsil tissue with CD63 antibody. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) HL-60, 2) U-251 MG, 3) THP-1, 4) A2058 and 5) A375 lysate with CD63 antibody. Predicted molecular weight: 25-60 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P08962
Localization Cytoplasm, cell surface and secreted
Applications Western blot : 1:1000
IHC (FFPE) : 1:25
Flow Cytometry : 1:25 per 1x10^6 cells
Limitations This CD63 antibody is available for research use only.
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Description

CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene.

Application Notes

Titration of the CD63 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 163-190 from the human protein was used as the immunogen for this CD63 antibody.

Storage

Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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