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Home >> Antibodies >> CD55 Antibody / DAF

CD55 Antibody / DAF [clone F4-29D9] (V2474)

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  Catalog No Formulation Size Price (USD)  
Image V2474-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2474-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2474SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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SDS-PAGE Analysis of Purified, BSA-Free CD55 Antibody (clone F4-29D9). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free CD55 Antibody (clone F4-29D9). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name F4-29D9
Purity Protein G affinity chromatography
UniProt P08174
Localization Cell surface
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Limitations This CD55 antibody is available for research use only.
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Description

Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). This mAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Application Notes

Optimal dilution of the CD55 antibody should be determined by the researcher.

Immunogen

Human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the CD55 antibody.

Storage

Store the CD55 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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