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Home >> Antibodies >> CD55 Antibody / DAF

CD55 Antibody / DAF [clone 143-30] (V2475)

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  Catalog No Formulation Size Price (USD)  
Image V2475-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2475-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2475SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Flow cytometry testing of PFA-fixed human HeLa cells with CD55 antibody (clone 143-30); Red=isotype control, Blue= CD55 antibody.
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Flow cytometry testing of PFA-fixed human HeLa cells with CD55 antibody (clone 143-30); Red=isotype control, Blue= CD55 antibody.
Immunofluorescent staining of PFA-fixed human HeLa cells with CD55 antibody (clone 143-30, green) and Reddot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free CD55 antibody (clone 143-30) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 143-30
Purity Protein G affinity chromatography
UniProt P08174
Localization Cell surface
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This CD55 antibody is available for research use only.
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Description

Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The mAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Application Notes

Optimal dilution of the CD55 antibody should be determined by the researcher.

Immunogen

PHA stimulated human PBL were used as the immunogen for the CD55 antibody.

Storage

Store the CD55 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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