CD54 Antibody DOD-9 / Major Rhinovirus Receptor Antibody [clone DOD-9] (RQ5121)

CD54 Antibody DOD-9 in Raji B Lymphoma Cells WB. Western blot testing of human Raji B lymphocyte lymphoma cell lysate with CD54 Antibody clone DOD-9 shows a strong predominant band at approximately 90 kDa. Although unmodified ICAM1/CD54 has a predicted molecular weight of approximately 58 kDa, extensive N-linked glycosylation produces mature forms that commonly migrate at approximately 75-115 kDa. The observed ~90 kDa band is therefore consistent with glycosylated ICAM1, the cell-surface protein recognized by this Major Rhinovirus Receptor Antibody.

CD54 Antibody DOD-9 in Human Cancer Cell Lines WB. Western blot testing of human HepG2, SiHa, K562, A431, Ramos, and HeLa cell lysates with CD54 Antibody clone DOD-9 shows predominant bands at approximately 90-110 kDa, with variable intensity among samples. Lanes 1-6: HepG2, SiHa, K562, A431, Ramos, and HeLa, respectively. Although unmodified ICAM1/CD54 has a predicted molecular weight of approximately 58 kDa, extensive N-linked glycosylation accounts for the higher apparent molecular weight. This Major Rhinovirus Receptor Antibody detects bands within the expected range for mature glycosylated ICAM1.

CD54 Antibody DOD-9 in Human Placenta IHC. Immunohistochemistry staining of FFPE human placenta tissue with CD54 Antibody clone DOD-9 shows prominent membranous staining of trophoblast cells lining the chorionic villi, with weaker cytoplasmic staining also visible. This Major Rhinovirus Receptor Antibody was applied at 1:50 overnight at 4oC following heat-induced epitope retrieval in EDTA buffer, pH 8.0, and blocking with 10% goat serum. HRP-based detection was performed using DAB chromogen.

CD54 Antibody DOD-9 in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with CD54 Antibody clone DOD-9 shows heterogeneous staining, with strong predominantly membranous immunoreactivity in localized tumor regions and weaker staining across much of the surrounding tissue. This Major Rhinovirus Receptor Antibody was applied at 1:50 overnight at 4oC following heat-induced epitope retrieval in EDTA buffer, pH 8.0, and blocking with 10% goat serum. HRP-based detection was performed using DAB chromogen.