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Home >> Antibodies >> CD50 Antibody / ICAM-3

CD50 Antibody / ICAM-3 [clone rICAM3/1019] (V8497)

  Catalog No Formulation Size Price (USD)  
Image V8497-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8497-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8497SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil with recombinant CD50 antibody (clone rICAM3/1019). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD50 antibody (clone rICAM3/1019) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rICAM3/1019
Purity Protein G affinity chromatography
UniProt P32942
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD50 antibody is available for research use only.
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Description

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

Application Notes

Optimal dilution of the CD50 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human ICAM3 protein was used as the immunogen for the CD50 antibody.

Storage

Store the CD50 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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