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Home >> Antibodies >> CD5 Antibody

CD5 Antibody (RQ4443)

  Catalog No Formulation Size Price (USD)  
Image RQ4443 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 389
Western blot testing of 1) human Jurkat, 2) human CCRF-CEM and 3) mouse NIH3T3 cell lysate with CD5 antibody at 0.5ug/ml. Expected molecular weight: 55-67 kDa depending on glycosylation level.
IHC testing of FFPE human tonsil tissue with CD5 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse spleen tissue with CD5 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat spleen tissue with CD5 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of mouse EL4 cells with CD5 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD5 antibody.
Immunofluorescent staining of FFPE rat brain with CD68 mAb (green), CD5 antibody (red) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P06127
Localization Cell membrane
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Flow Cytometry : 1-3ug/10^6 cells
Immunofluorescence : 1ug/ml
Limitations This CD5 antibody is available for research use only.
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Description

CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the CD5 antibody should be determined by the researcher.

Immunogen

Human CD5 recombinant protein (amino acids R25-L495) was used as the immunogen for the CD5 antibody.

Storage

After reconstitution, the CD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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