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Home >> Antibodies >> CD5 Antibody

CD5 Antibody [clone CD5/54/F6] (V3120)

  Catalog No Formulation Size Price (USD)  
Image V3120-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V3120-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V3120SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V3120IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with CD5 antibody (clone CD5/54/F6).
Western blot testing of MOLT4 cell lysate with CD5 antibody (clone CD5/54/F6). Observed molecular weight 55~67 kDa depending on glycosylation level.
SDS-PAGE Analysis of Purified, BSA-Free CD5 Antibody (clone CD5/54/F6). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CD5/54/F6
Purity Protein G affinity chromatography
UniProt P06127
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CD5 antibody is available for research use only.
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Description

Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Application Notes

The optimal dilution of the CD5 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A peptide from the intracellular region of the human protein was used as the immunogen for this CD5 antibody.

Storage

Store the CD5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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