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Home >> Antibodies >> CD5 Antibody

CD5 Antibody [clone B-B8] (V2960)

  Catalog No Formulation Size Price (USD)  
Image V2960-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2960-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2960SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name B-B8
Purity Protein G affinity chromatography
UniProt P06127
Localization Cell surface
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD5 antibody is available for research use only.
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Description

Recognizes a 67kDa transmembrane protein which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Note that this mAb is not suitable for frozen tissues.

Application Notes

Optimal dilution of the CD5 antibody should be determined by the researcher.

Immunogen

T cells activated with PHA were used as the immunogen for the CD5 antibody.

Storage

Store the CD5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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