CD47 Antibody B6H12.2 / OA3 Antibody [clone B6H12.2] (V2069)

CD47 Antibody B6H12.2 in Human MCF7 Breast Cancer Cells FACS. Flow cytometry testing of PFA-fixed human MCF7 breast cancer cells with CD47 Antibody clone B6H12.2. The antibody-stained population (blue) shows a pronounced rightward fluorescence shift relative to the isotype control (red), with clear separation between the distributions. The strong shift demonstrates detection of endogenous CD47 in fixed and permeabilized MCF7 cells and supports cellular recognition with the OA3 Antibody.

CD47 Antibody B6H12.2 in Human MCF7 Breast Cancer Cells IF. Immunofluorescence staining of PFA-fixed human MCF7 breast cancer cells with CD47 Antibody clone B6H12.2 (green) and RedDot nuclear stain (red). Green fluorescence is distributed prominently around the cell periphery, with additional intracellular signal visible throughout the cells, while nuclei are delineated by the red counterstain. The staining demonstrates cellular detection of CD47 with the OA3 Antibody.

CD47 Antibody B6H12.2 in Human Jurkat T Cells IF. Immunofluorescence staining of PFA-fixed human Jurkat T cells with CD47 Antibody clone B6H12.2 (green) and RedDot nuclear stain (red). Strong green fluorescence is visible throughout the rounded Jurkat cells and around their periphery, with the red counterstain identifying nuclei. This distinct cellular staining demonstrates recognition of endogenous CD47 in a human T-cell leukemia model with the OA3 Antibody.

CD47 Antibody B6H12.2 Detects Live Human Jurkat T Cells FACS. Flow cytometry testing of live human Jurkat T cells with CD47 Antibody clone B6H12.2. The antibody-stained population (blue) shows a strong rightward fluorescence shift relative to the isotype control (red), demonstrating recognition of native CD47 exposed on the surface of intact, non-fixed cells. This live-cell detection is particularly relevant for the cell-surface biology of CD47 and supports recognition of native OA3 Antibody epitopes.

CD47 Antibody B6H12.2 Shows Cell-Surface Staining of Live Jurkat Cells IF. Immunofluorescence staining of live human Jurkat T cells with CD47 Antibody clone B6H12.2 (green). Strong green fluorescence outlines the rounded cells, producing prominent peripheral staining consistent with localization of CD47 at the plasma membrane. Detection without fixation or permeabilization demonstrates recognition of extracellularly accessible CD47 on intact cells and provides complementary live-cell validation for the OA3 Antibody.

CD47 Antibody B6H12.2 Western Blot of Human Brain. Western blot testing of human brain lysate with CD47 Antibody clone B6H12.2. Immunoreactivity is detected across approximately 35-60 kDa rather than as a single sharp band at the predicted molecular weight of approximately 35 kDa. This broader and higher apparent molecular weight is consistent with the extensive glycosylation of native CD47, which can substantially alter its electrophoretic migration. The observed target-range signal supports western blot detection with the OA3 Antibody.

Purified CD47 Antibody B6H12.2 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free CD47 Antibody clone B6H12.2 provides a quality-control assessment of antibody integrity and purity. The non-reduced preparation shows predominantly intact immunoglobulin near 150 kDa, while reducing conditions produce the expected antibody heavy and light chains at approximately 50 kDa and 25 kDa. The characteristic electrophoretic profile supports the integrity of the purified OA3 Antibody preparation.