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Home >> Antibodies >> CD47 Antibody

CD47 Antibody [clone CD47/2937] (V7536BTN)

  Catalog No Formulation Size Price (USD)  
Image V7536BTN 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 500 ul 519
Microvalidated
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IHC staining of FFPE human prostate carcinoma with biotinylated CD47 antibody (clone CD47/2937). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with biotinylated CD47 antibody (clone CD47/2937). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD47 antibody (clone CD47/2937). These results demonstrate the foremost specificity of the CD47/2937 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD47 antibody (clone CD47/2937) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Biotin Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2c, kappa
Clone Name CD47/2937
Purity Protein G affinity chromatography
UniProt Q08722
Localization Cell surface, cytoplasmic
Applications Western blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 minutes at RT
Limitations This CD47 antibody is available for research use only.
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Description

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Application Notes

Optimal dilution of the CD47 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 18-135 from the human protein was used as the immunogen for the CD47 antibody.

Storage

Store the CD47 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

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