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Home >> Antibodies >> CD45RA Antibody

CD45RA Antibody [clone PTPRC/8124R] (V4764)

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  Catalog No Formulation Size Price (USD)  
Image V4764-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4764-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4764SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with CD45RA antibody (clone PTPRC/8124R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD45RA antibody (clone PTPRC/8124R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC testing of FFPE human brain tissue with CD45RA antibody (clone PTPRC/8124R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PTPRC/8124R
Purity Protein A/G affinity
UniProt P08575
Localization Cell surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CD45RA antibody is available for research use only.
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Description

CD45RA antibody detects CD45RA, a splice variant of the Leukocyte common antigen encoded by the PTPRC gene. CD45 functions as a receptor-type protein tyrosine phosphatase essential for signaling through the T cell receptor and B cell receptor pathways. CD45RA antibody identifies the RA isoform, which is prominently expressed on naive T cells and certain B cell populations, serving as a widely used immunophenotypic marker in immunology research.

CD45RA differs from other CD45 isoforms by inclusion of exon A in its extracellular domain. This structural difference influences glycosylation patterns and may affect receptor interactions at the cell surface. The intracellular phosphatase domains of CD45 dephosphorylate inhibitory tyrosine residues on Src family kinases, thereby promoting antigen receptor signaling. CD45RA antibody is frequently applied in studies examining T cell maturation, thymic development, and immune reconstitution following therapy.

Alterations in CD45 splicing and expression are linked to hematologic malignancies and immune disorders. CD45RA antibody provides a reliable approach for studying immune cell differentiation and leukocyte lineage characterization in research settings.

Application Notes

Optimal dilution of the CD45RA antibody should be determined by the researcher.

Immunogen

Recombinant full-length human PTPRC protein was used as the immunogen for the CD45RA antibody.

Storage

Aliquot the CD45RA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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