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Home >> Antibodies >> CD44 Antibody / HCAM

CD44 Antibody / HCAM [clone rHCAM/6449] (V8829)

  Catalog No Formulation Size Price (USD)  
Image V8829-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8829-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8829SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human esophagus tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using CD44 antibody (clone rHCAM/6449) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rHCAM/6449
Purity Protein A/G affinity
UniProt P16070
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD44 antibody is available for research use only.
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Description

The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding.

Application Notes

Optimal dilution of the CD44 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CD44 protein was used as the immunogen for the CD44 antibody.

Storage

Aliquot the CD44 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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