CD44 Antibody for FACS Jurkat / T Cell Surface Marker Antibody (R32994)

CD44 Antibody Multi-Species WB. Western blot analysis of CD44 / CD44 antigen expression in multiple lysates using rabbit polyclonal antibody at 0.5 ug/ml. Lane 1: human HeLa cell lysate, Lane 2: human 22RV1 cell lysate, Lane 3: rat spleen lysate, Lane 4: rat thymus lysate, Lane 5: mouse spleen lysate. A band is detected at approximately 80-95 kDa, consistent with the predicted molecular weight of CD44 (CD44 / CD44 antigen). CD44 is a glycosylated transmembrane protein, and the observed band range reflects variable glycosylation of the extracellular domain across species and tissue types. The consistent detection across human, rat, and mouse samples supports CD44 expression in both epithelial and lymphoid tissues and aligns with its role as a broadly expressed cell surface adhesion molecule.

CD44 Antibody Colon Cancer IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE human colon cancer tissue using rabbit polyclonal antibody at 1 ug/ml. Membranous HRP-DAB brown staining is observed in tumor epithelial cells, outlining cell borders and highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates heterogeneous intensity across tumor regions and supports its use for evaluating CD44 distribution, tumor cell organization, and microenvironment-associated expression in colorectal carcinoma tissue. Heat induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling prior to testing.

CD44 Antibody Placenta IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE human placental tissue using rabbit polyclonal antibody at 1 ug/ml. Membranous HRP-DAB brown staining is observed in trophoblast and stromal-associated cells, highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates regional variation between villous structures and stromal compartments and supports its use for evaluating CD44 distribution and cellular organization in placental tissue. Heat induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling prior to testing.

CD44 Antibody Rat Kidney IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE rat kidney tissue using rabbit polyclonal antibody at 1 ug/ml. Membranous HRP-DAB brown staining is observed in tubular epithelial cells and along vascular-associated structures, highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates regional variation across renal compartments, including tubular and interstitial areas, and supports its use for evaluating CD44 distribution and cellular organization in kidney tissue. Heat induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling prior to testing.

CD44 Antibody Mouse Kidney IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE mouse kidney tissue using rabbit polyclonal antibody at 1 ug/ml. Membranous HRP-DAB brown staining is observed in tubular epithelial cells and along vascular-associated structures, highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates widespread distribution across renal tubules with additional signal in interstitial regions, supporting its use for evaluating CD44 expression and cellular organization in kidney tissue. Heat induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling prior to testing.

GNB1 Antibody Mouse Lymph Tissue IF. Immunofluorescence analysis of GNB1 expression in FFPE mouse lymph tissue using antibody at 1 ug/ml (green) with DAPI nuclear stain (blue). Diffuse cytoplasmic and membrane-associated fluorescence is observed throughout lymphoid cell populations, highlighting widespread GNB1 expression within the tissue. The staining pattern demonstrates dense cellular distribution consistent with lymphoid architecture and supports its use for visualizing GNB1 localization in immune cell-rich environments. Heat induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min.

GNB1 Antibody Mouse Lymph Tissue IF. Immunofluorescence analysis of GNB1 expression in FFPE mouse lymph tissue using antibody at 1 ug/ml (green) with DAPI nuclear stain (blue). Strong cytoplasmic fluorescence is observed in densely packed lymphoid cell populations, with signal distributed throughout the tissue and highlighting cellular architecture within lymphoid regions. The staining pattern supports GNB1 localization in immune cell-rich environments and is consistent with its role in intracellular signaling. Heat induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min.

CD44 Antibody Jurkat Cell FACS. Flow cytometry analysis of CD44 / CD44 antigen expression in human Jurkat cells using rabbit polyclonal antibody at 1 ug per 10^6 cells following blocking with goat sera. The CD44 antibody-stained population (blue) demonstrates a pronounced right-shift compared to cells alone (red) and isotype control (green), indicating strong cell surface expression of CD44. The clear separation between populations supports its use for identifying, gating, and quantifying CD44-positive T cell populations in flow cytometry assays.