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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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CD44 antigen (CD44) is a transmembrane glycoprotein that functions as a receptor for hyaluronic acid and mediates cell adhesion, migration, and extracellular matrix interactions. It is widely expressed on immune cells, including lymphocytes, where it plays a central role in cell-cell communication and spatial organization within tissues. CD44 Antibody for IF / Lymphoid Tissue Immunofluorescence Antibody is designed to detect CD44 expression in immunofluorescence applications, enabling high-resolution visualization of cell surface localization and spatial distribution within lymphoid tissue sections.
CD44 antibody, also referred to as CD44 antigen antibody or Hermes antigen antibody, recognizes a cell surface glycoprotein involved in immune cell adhesion and trafficking. In lymphoid tissues such as tonsil and lymph node, CD44 expression is associated with dense immune cell populations and organized tissue architecture. Immunofluorescence detection allows precise visualization of CD44-positive cells within these compartments, supporting analysis of tissue structure and immune cell localization at the cellular level.
Functionally, CD44 mediates interactions with hyaluronic acid and other extracellular matrix components, facilitating immune cell migration and positioning within tissue microenvironments. In immunofluorescence applications, CD44 staining typically appears as membrane-associated fluorescence outlining cell borders, with signal intensity reflecting local cell density and tissue organization. This CD44 Antibody for IF is particularly suited for examining lymphoid architecture, immune cell distribution, and membrane localization of CD44 in both human and rodent tissues.
CD44 expression in human tonsil and rat lymph tissue demonstrates conserved localization across species and highlights its role as a broadly expressed immune cell marker. Detection in these systems enables comparative analysis of lymphoid structure and cellular organization. The use of a rabbit polyclonal antibody provides sensitive detection of CD44 across heterogeneous immune cell populations in immunofluorescence assays.
Structurally, CD44 consists of an extracellular ligand-binding domain, a transmembrane segment, and a cytoplasmic tail involved in intracellular signaling. Alternative splicing generates multiple isoforms, while glycosylation contributes to structural and functional diversity. An antibody targeting CD44 is suitable for detecting this cell surface protein in immunofluorescence and related imaging applications, with strong utility for visualizing immune cell organization within tissue environments.
This CD44 antibody is part of a broader CD44 antibody panel offered by NSJ Bioreagents.
The stated application concentrations are suggested starting amounts. Titration of the CD44 Antibody for IF / Lymphoid Tissue Immunofluorescence Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the N-terminus of human CD44 (RFAGVFHVEKNGRYSISRTEAADLCKAF) was used as the immunogen for this CD44 antibody.
After reconstitution, the CD44 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
CD44 antibody, CD44 immunofluorescence antibody, CD44 lymphoid tissue antibody, CD44 antigen antibody, Hermes antigen antibody
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