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Home >> Antibodies >> CD44 Antibody

CD44 Antibody (R31746)

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  Catalog No Formulation Size Price (USD)  
Image R31746 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human tonsil with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human tonsil with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat lymph tissue with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human intestinal cancer with CD44 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human U-87 MG, 3) rat PC-12 and 4) rat C6 cell lysate with CD44 antibody. Predicted molecular weight ~82 kDa, but may be observed at higher molecular weights due to glycosylation.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 960
Localization Cell surface, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Limitations This CD44 antibody is available for research use only.
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Description

Cluster of differentiation 44 is also known as LHR or MC56. CD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for its gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the CD44 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human CD44 (RFAGVFHVEKNGRYSISRTEAADLCKAF) was used as the immunogen for this CD44 antibody.

Storage

After reconstitution, the CD44 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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