CD44 Antibody for FACS HL60 / Leukocyte Marker Antibody (R30197)

CD44 Antibody Laryngeal Squamous Cell Carcinoma IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE human laryngeal squamous cell carcinoma tissue using antibody. Strong membranous HRP-DAB brown staining is observed in malignant squamous epithelial cells, outlining cell borders and highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates cohesive tumor architecture and supports its use for evaluating epithelial organization and CD44-associated cell adhesion in squamous cell carcinoma. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing.

CD44 Antibody Rectal Cancer IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE human rectal cancer tissue using antibody. Membranous HRP-DAB brown staining is observed in tumor epithelial cells, outlining cell borders and highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates heterogeneous intensity across tumor regions and supports its use for evaluating CD44 distribution, tumor cell organization, and microenvironment-associated expression in colorectal carcinoma tissue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing.

CD44 Antibody Rat Lung IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE rat lung tissue using antibody. Membranous HRP-DAB brown staining is observed in scattered epithelial and interstitial cells, highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates regional distribution across alveolar structures and supports its use for evaluating CD44 expression and cellular organization in lung tissue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing.

CD44 Antibody Human and Rat Cell Line WB. Western blot analysis of CD44 / CD44 antigen expression in multiple lysates using antibody. Lane 1: human HeLa cell lysate, Lane 2: rat PC-12 cell lysate. A band is detected at approximately 80-95 kDa, consistent with the predicted molecular weight of CD44 (CD44 / CD44 antigen). CD44 is a glycosylated transmembrane protein, and higher apparent molecular weight bands may be observed due to variable glycosylation of the extracellular domain. The detected signal supports CD44 expression across species and is consistent with its role as a broadly expressed cell surface adhesion molecule.

CD44 Antibody HL60 Cell FACS. Flow cytometry analysis of CD44 / CD44 antigen expression in human HL60 cells using rabbit polyclonal antibody at 1 ug per million cells following blocking with goat sera. The CD44 antibody-stained population (blue) demonstrates a clear right-shift compared to cells alone (red) and isotype control (green), indicating strong cell surface expression of CD44. The distinct separation between populations supports its use for identifying, gating, and quantifying CD44-positive leukocyte populations in flow cytometry assays.