CD44 Antibody Clone Hermes-3 / Leukocyte Marker Antibody [clone Hermes-3] (F40263)

CD44 Antibody Human Cell Line WB. Western blot analysis of CD44 / CD44 antigen expression in human HeLa cell lysate using mouse monoclonal antibody clone Hermes-3. A band is detected at approximately 80-95 kDa, consistent with the predicted molecular weight of CD44 (CD44 / CD44 antigen). CD44 is a glycosylated transmembrane protein, and the observed band reflects the core protein with potential variation in apparent molecular weight due to glycosylation of the extracellular domain. The detected signal supports CD44 expression in HeLa cells and is consistent with its role as a cell surface adhesion molecule.

CD44 Antibody Esophagus Carcinoma IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE human esophagus carcinoma tissue using mouse monoclonal antibody clone Hermes-3. Strong membranous HRP-DAB brown staining is observed in malignant epithelial cells, outlining cell borders and highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates cohesive tumor architecture and supports its use for evaluating epithelial organization and CD44-associated cell surface interactions in esophageal carcinoma tissue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling at RT before testing.

CD44 Antibody HeLa Cell IF. Immunofluorescence analysis of CD44 / CD44 antigen expression in human HeLa cells using mouse monoclonal antibody clone Hermes-3 (red) with DAPI nuclear stain (blue). Strong membrane-associated fluorescence is observed outlining cell borders, highlighting cell surface localization consistent with CD44 expression. The staining pattern demonstrates clear cell perimeter labeling and supports its use for visualizing CD44-mediated cell surface interactions and cellular morphology in cultured human cells.

CD44 Antibody HeLa Cell FACS. Flow cytometry analysis of CD44 / CD44 antigen expression in human HeLa cells using mouse monoclonal antibody clone Hermes-3. The right-shifted histogram (red) demonstrates increased fluorescence intensity compared to the negative control population (blue), indicating positive cell surface expression of CD44. The clear separation between populations supports its use for detecting and quantifying CD44-expressing cells and analyzing cell surface marker distribution in cultured human cells.

CD44 Antibody Skin IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE human skin tissue using mouse monoclonal antibody clone Hermes-3. Strong membranous HRP-DAB brown staining is observed in stratified squamous epithelial cells, outlining cell borders and highlighting organized epithelial layering consistent with CD44 expression. The staining pattern emphasizes cell surface localization within epidermal layers and supports its use for evaluating epithelial structure and CD44-associated cell adhesion in skin tissue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling at RT before testing.

CD44 Antibody Skin Tissue IHC. Immunohistochemistry analysis of CD44 / CD44 antigen expression in FFPE human skin tissue using mouse monoclonal antibody clone Hermes-3. Membranous HRP-DAB brown staining is observed throughout stratified squamous epithelial layers, clearly outlining cell borders and demonstrating organized epithelial architecture consistent with CD44 expression. The staining pattern highlights uniform cell surface localization and supports its use for evaluating epithelial structure and CD44-associated cell adhesion in skin tissue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling at RT before testing.

CD44 Antibody HeLa and HUVEC Human Cell Line WB. Western blot analysis of CD44 / CD44 antigen expression in human cell lysates using mouse monoclonal antibody clone Hermes-3. Lane 1: HeLa cell lysate, Lane 2: HUVEC cell lysate. A band is detected at approximately 80-95 kDa, consistent with the predicted molecular weight of CD44 (CD44 / CD44 antigen). CD44 is a glycosylated transmembrane protein, and the observed band reflects the core protein with potential variation in apparent molecular weight due to glycosylation of the extracellular domain. The detected signal supports CD44 expression in both epithelial-derived and endothelial cell types, consistent with its role as a broadly expressed cell surface adhesion molecule.