CD44 Antibody / Cancer Stem Cell Marker Antibody [clone HCAM/918] (V3011)

Description

CD44 antigen (CD44), also known as homing cell adhesion molecule (HCAM), is a transmembrane glycoprotein that functions as a receptor for hyaluronic acid and mediates cell adhesion, migration, and extracellular matrix interactions. It is widely expressed on epithelial and immune cells, where it contributes to cell surface communication and tissue organization. CD44 Antibody / Cancer Stem Cell Marker Antibody (clone HCAM/918) is designed to detect CD44 expression in formalin-fixed, paraffin-embedded tissues and related applications, with particular relevance for identifying cancer stem cell-associated subpopulations and evaluating cellular heterogeneity within tumor samples.

CD44 antibody, also referred to as HCAM antibody or Hermes antigen antibody, recognizes a cell surface glycoprotein that is strongly associated with cancer stem cell biology across multiple tumor types. CD44-positive subpopulations have been reported to exhibit enhanced capacity for self-renewal, differentiation, and tumor initiation, making CD44 a widely used marker for identifying stem-like cells within heterogeneous tumor environments. Clone HCAM/918 is supported by multi-application testing and published data, providing a robust tool for investigating CD44 expression in the context of tumor hierarchy and cellular subpopulation analysis.

Functionally, CD44 mediates interactions with hyaluronic acid and other extracellular matrix components, supporting cell adhesion, migration, and microenvironmental signaling. These processes are particularly relevant in cancer stem cells, which depend on niche interactions and spatial positioning within tumor tissue to maintain stem-like properties. In immunohistochemistry applications, CD44 staining typically presents as membranous HRP-DAB signal outlining cell borders, with heterogeneous intensity across tumor regions. Distinct subsets of strongly positive cells may be observed within broader tumor populations, consistent with enrichment of CD44-expressing subpopulations. This CD44 Antibody clone HCAM/918 is particularly suited for identifying cellular subsets, evaluating tumor heterogeneity, and assessing spatial organization of stem-like populations.

CD44 expression is frequently detected in tumor epithelial cells and may be enriched in specific regions associated with active growth, differentiation, or microenvironmental interaction. Variability in staining intensity and distribution can reflect differences in cellular state, organization, and functional role within the tumor. Detection of CD44 in these contexts supports studies of tumor biology, cellular hierarchy, and the relationship between stem-like populations and overall tissue structure.

Structurally, CD44 consists of an extracellular ligand-binding domain, a transmembrane segment, and a cytoplasmic tail involved in intracellular signaling and cytoskeletal interactions. Alternative splicing generates multiple isoforms, while glycosylation contributes to structural and functional diversity. An antibody targeting CD44 is suitable for detecting membrane-associated expression and studying cancer stem cell-associated biology, cellular heterogeneity, and tissue organization in a wide range of research applications.

This CD44 antibody is part of a broader CD44 antibody panel offered by NSJ Bioreagents.

Application Notes

Optimal dilution of the CD44 Antibody / Cancer Stem Cell Marker Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the CD44 antibody.

Storage

Store the CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

CD44 antibody, CD44 cancer stem cell antibody, HCAM antibody, CD44 antigen antibody, Hermes antigen antibody