• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> CD43 Antibody

CD43 Antibody [clone SPN/3388] (V7576)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V7576-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
Image
V7576-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
Image
V7576SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Image
V7576IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Bulk quote request
IHC staining of FFPE human spleen with CD43 antibody (clone SPN/3388). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTAfor 10-20 min and allow to cool before testing.
›
IHC staining of FFPE human spleen with CD43 antibody (clone SPN/3388). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Flow cytometry testing of PFA-fixed human K562 cells with CD43 antibody (clone SPN/3388); Red=isotype control, Blue= CD43 antibody.
Immunofluorescence staining of PFA-fixed human K562 cells with CD43 antibody (green, clone SPN/3388) and NucSpot Live 650 (red).
Western blot testing of human K562 cell lysate with CD43 antibody (clone SPN/3388). Expected molecular weight: 45-135 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free CD43 antibody (clone SPN/3388) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPN/3388
Purity Protein G affinity chromatography
UniProt P16150
Localization Cell surface
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CD43 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Application Notes

Optimal dilution of the CD43 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human SPN protein was used as the immunogen for the CD43 antibody.

Storage

Store the CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all CD43 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.