GPIIb Antibody / Platelet Glycoprotein IIb Antibody (RQ4069)

GPIIb Antibody Detection in HL60 Human Promyelocytic Leukemia Cells by FACS. Flow cytometry analysis of human HL60 promyelocytic leukemia cells using GPIIb Antibody at 1 ug/million cells shows a pronounced rightward shift of the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red). The three populations are well separated, with the CD41-positive signal extending approximately one log beyond the negative control population. This result demonstrates detection of cell-associated GPIIb/CD41 in HL60 cells using this Platelet Glycoprotein IIb Antibody.

GPIIb Antibody Detection in U-937 Human Histiocytic Lymphoma Cells by FACS. Flow cytometry analysis of human U-937 histiocytic lymphoma cells using GPIIb Antibody at 1 ug/million cells shows a clear rightward shift of the antibody-stained population (blue) compared with the isotype control (green) and cells-alone control (red). The positive population is distinctly separated from the unstained cells, although some overlap with the isotype distribution is visible. This result demonstrates detection of GPIIb/CD41 in U-937 cells using this Platelet Glycoprotein IIb Antibody.

GPIIb Antibody Staining in Human Rectal Carcinoma by IHC. Immunohistochemistry of FFPE human rectal carcinoma tissue using GPIIb Antibody at 1 ug/ml shows localized brown staining distributed among cells and structures within the tumor tissue. Signal is heterogeneous across the field and readily distinguishable from the blue hematoxylin-counterstained nuclei. Heat-induced epitope retrieval was performed by steaming the tissue sections in pH 6 citrate buffer for 20 min followed by cooling before testing. The observed staining demonstrates detection of CD41 using this Platelet Glycoprotein IIb Antibody.

GPIIb Antibody Staining in Human Breast Carcinoma by IHC. Immunohistochemistry of FFPE human breast carcinoma tissue using GPIIb Antibody at 1 ug/ml shows relatively sparse, localized brown staining against predominantly blue hematoxylin-counterstained tissue. The staining is concentrated in discrete cells and cellular structures rather than broadly distributed throughout the epithelial compartment. Heat-induced epitope retrieval was performed by steaming the sections in pH 6 citrate buffer for 20 min followed by cooling before testing. This localized staining demonstrates detection of CD41 using this Platelet Glycoprotein IIb Antibody.

GPIIb Antibody Detection in Human, Rat and Mouse Samples by WB. Western blot testing with GPIIb Antibody at 0.5 ug/ml was performed using human K562 cells (lane 1), human Raji cells (lane 2), rat testis (lane 3), rat brain (lane 4) and mouse brain (lane 5). Prominent bands migrate at approximately 150-170 kDa in lanes 1, 3 and 5, with substantially weaker signal in lanes 2 and 4. ITGA2B is extensively glycosylated, which can increase its apparent molecular weight above the calculated mass of the polypeptide. The cross-species signals demonstrate recognition of Platelet Glycoprotein IIb/CD41 by this GPIIb Antibody.

GPIIb Antibody Staining in Human Tonsil by IF. Immunofluorescence staining of FFPE human tonsil tissue using GPIIb Antibody at 1 ug/ml shows green fluorescence distributed throughout portions of the lymphoid tissue, with heterogeneous signal surrounding a comparatively less reactive central region. Heat-induced epitope retrieval was performed by boiling the sections in pH 6, 10 mM citrate buffer for 10-20 min followed by cooling. The staining demonstrates detection of CD41-associated structures in human tonsil using this Platelet Glycoprotein IIb Antibody.

GPIIb Antibody Staining in Mouse Lymph Tissue by IF. Immunofluorescence staining of FFPE mouse lymph tissue using GPIIb Antibody at 1 ug/ml shows punctate and cellular green fluorescence distributed throughout the tissue, with DAPI-counterstained nuclei shown in blue. Signal is heterogeneous, with multiple discrete CD41-positive structures visible among the surrounding cells. Heat-induced epitope retrieval was performed by steaming the sections in pH 6 citrate buffer for 20 min. This cross-species staining demonstrates recognition of mouse CD41 using this Platelet Glycoprotein IIb Antibody.