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Home >> Antibodies >> CD4 Antibody

CD4 Antibody [clone CD4/7142] (V9753)

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  Catalog No Formulation Size Price (USD)  
Image V9753-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9753-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9753SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human lymph node with CD4 antibody (clone CD4/7142) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lymph node with CD4 antibody (clone CD4/7142) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD4 antibody (clone CD4/7142). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2c, kappa
Clone Name CD4/7142
Purity Protein A/G affinity
UniProt P01730
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD4 antibody is available for research use only.
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Description

Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Application Notes

Optimal dilution of the CD4 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 200-400 was used as the immunogen for the CD4 antibody.

Storage

Aliquot the CD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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