- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD3e Antibody / Microarray Specificity Validated Antibody [clone C3e/2479] (V3752)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V3752-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V3752-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V3752SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | C3e/2479 |
| Purity | Protein G affinity chromatography |
| UniProt | P07766 |
| Localization | Cell surface and cytoplasmic |
| Applications | ELISA : 2-4ug/ml (order BSA/azide-free format) Western Blot : 1-2ug/ml |
| Limitations | This CD3e antibody is available for research use only. |
|
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CD3 epsilon (CD3E) is a transmembrane signaling component of the T-cell receptor (TCR) complex that is consistently expressed in T lymphocytes and plays a central role in antigen recognition and downstream activation. CD3e Antibody / Microarray Specificity Validated Antibody is designed to detect CD3 Epsilon / CD3E with a high degree of confidence, supported by protein microarray-based specificity validation. CD3e antibody, also known as CD3 epsilon antibody or CD3E antibody, is widely recognized as a pan-T cell marker antibody and is routinely used in studies of T-cell biology, immune signaling, and lymphocyte characterization.
CD3 epsilon forms part of the CD3 signaling complex together with CD3 gamma, CD3 delta, and CD3 zeta chains, which associate with the TCR alpha-beta or gamma-delta heterodimer at the cell surface. This complex is essential for initiating intracellular signaling cascades that regulate T-cell activation, proliferation, and differentiation. Because CD3 epsilon is a core component of this signaling machinery, accurate and specific detection is critical for interpreting T-cell-related data across a wide range of experimental systems.
This CD3e Antibody / Microarray Specificity Validated Antibody is uniquely positioned for applications where antibody specificity is a primary concern. Clone C3e/2479 has been evaluated using protein microarray technology, enabling comprehensive screening of antibody binding against thousands of human proteins in parallel. This large-scale approach provides a detailed specificity profile, confirming selective recognition of CD3 epsilon while minimizing cross-reactivity with non-target proteins. Such validation extends beyond traditional single-target testing and offers a broader assessment of antibody performance in complex biological environments.
Protein microarray specificity validation is particularly valuable in experiments involving multiplex detection, closely related protein families, or heterogeneous samples where non-specific binding can lead to misleading results. By demonstrating selective binding across a wide protein landscape, this CD3e antibody provides an additional layer of assurance that detected signals correspond to true CD3 epsilon expression. This is especially important in immune profiling and signaling studies, where accurate identification of T-cell populations underpins downstream analysis.
The use of a microarray-validated CD3e antibody can reduce the risk of false-positive signals and improve reproducibility across experiments. In complex lysates, tissue samples, or multi-marker panels, even low-level cross-reactivity can compromise data interpretation. The specificity profile of clone C3e/2479 supports cleaner signal detection, improved confidence in results, and more reliable comparison across experimental conditions.
As a mouse monoclonal antibody, clone C3e/2479 provides consistent epitope recognition and batch-to-batch reproducibility. When combined with protein microarray specificity validation, this format delivers a high-confidence reagent for detecting CD3 epsilon. CD3e Antibody / Microarray Specificity Validated Antibody is well suited for research applications requiring precise target recognition, supporting accurate analysis of T-cell biology, immune signaling, and cellular composition.
A full range of CD3e antibody reagents for immunohistochemistry, western blot, and flow cytometry is available on our CD3e Antibody collection page.
Optimal dilution of the CD3e Antibody / Microarray Specificity Validated Antibody should be determined by the researcher.
A portion of amino acids 23-119 were used as the immunogen for the CD3e Antibody / Microarray Specificity Validated Antibody.
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CD3E antibody, CD3 epsilon specificity validated antibody, CD3 microarray validated antibody, CD3 antigen epsilon chain antibody, T cell marker specificity antibody
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