CD3D Antibody / CD3 delta subunit [clone CD3D/13089R] (V6031)

Immunohistochemistry analysis of CD3D/CD3 delta subunit antibody (clone CD3D/13089R) in human tonsil tissue. Formalin-fixed, paraffin-embedded tonsil demonstrates strong membranous HRP-DAB brown staining in interfollicular T lymphocytes, consistent with CD3 complex expression in mature T cells. Germinal center B cell areas show minimal staining, highlighting the expected T cell-restricted distribution pattern. Hematoxylin counterstain delineates nuclear morphology and tonsillar architecture. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.

Western blot analysis of CD3D/CD3 delta subunit antibody (clone CD3D/13089R) in human Jurkat cell lysate. A distinct band is observed at approximately 20-25 kDa, consistent with the predicted molecular weight of the CD3 delta subunit. The detected signal corresponds to endogenous CD3 delta expressed in T lymphoblastic Jurkat cells. Molecular weight markers are indicated in kDa on the left.

Western blot analysis of CD3D/CD3 delta subunit antibody (clone CD3D/13089R) in human Jurkat and MOLT-4 cell lysates. A single band is observed at approximately 20-25 kDa in both lanes, consistent with the predicted molecular weight of the CD3 delta subunit. Comparable band intensity in Jurkat and MOLT-4 cells reflects endogenous expression of CD3 delta in T lymphoblastic cell lines. Molecular weight markers are indicated in kDa on the left.