CD3D Antibody Rabbit Monoclonal / CD3 delta [clone IDE-3] (RQ5046)

Immunofluorescence analysis of CD3D antibody in human Jurkat cells. CD3D antibody (green) demonstrates prominent membrane-associated fluorescence outlining the cell surface, consistent with CD3 delta localization within the T cell receptor CD3 complex. Nuclear counterstain with DAPI (blue) highlights cell nuclei and confirms cellular integrity. The staining pattern is consistent with surface expression of CD3 delta in T lymphoblastic Jurkat cells.

Immunohistochemistry analysis of CD3D antibody in human spleen tissue. Paraffin-embedded spleen demonstrates membranous HRP-DAB brown staining in numerous lymphocytes within periarteriolar lymphoid sheaths and surrounding white pulp regions, consistent with CD3 delta expression in mature T cells. Red pulp areas show comparatively fewer positive cells, reflecting the expected distribution of T lymphocytes. Hematoxylin counterstain highlights nuclear morphology and splenic architecture.

Western blot testing of human Jurkat cell lysate with CD3D antibody. Predicted molecular weight ~19 kDa.

Western blot analysis of CD3D antibody in human Jurkat and MOLT-4 cell lysates. A prominent band is observed at approximately 20-22 kDa in both lanes, consistent with the predicted molecular weight of CD3 delta, which is approximately 19 kDa based on amino acid sequence. The slight upward shift in apparent molecular weight is commonly observed in SDS-PAGE and may reflect electrophoretic migration differences or post-translational modifications. Strong signal intensity in both Jurkat and MOLT-4 cells is expected due to high endogenous expression of CD3 delta in T lymphoblastic cell lines. Molecular weight markers are indicated in kDa on the left.