CD38 Antibody / Tumor Immune Microenvironment Marker Antibody [clone rCD38/6447] (V9296)

CD38 Antibody human Raji lysate WB. Western blot analysis of CD38 expression in human Raji cell lysate using CD38 antibody clone rCD38/6447. Lane 1: human Raji cell lysate. A band is detected at approximately 40–45 kDa, consistent with the predicted molecular weight of CD38, with higher apparent molecular weight reflecting glycosylation of this cell surface protein and aligning with expression in B cell-derived lines. The detection profile supports its use in studies of immune cell presence within the tumor microenvironment.

CD38 Antibody human tonsil tissue IHC. Immunohistochemistry analysis of CD38 expression in FFPE human tonsil tissue using CD38 antibody clone rCD38/6447 at 2 ug/ml. Strong membranous and cytoplasmic HRP-DAB brown staining highlights numerous plasma cells and activated lymphocytes within interfollicular regions and surrounding germinal centers, demonstrating dense immune cell distribution. The staining pattern is consistent with detection of immune cell populations within lymphoid tissue architecture, with clear contrast against predominantly negative background lymphocytes. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

CD38 Antibody human prostate adenocarcinoma tissue IHC. Immunohistochemistry analysis of CD38 expression in FFPE human prostate adenocarcinoma tissue using CD38 antibody clone rCD38/6447 at 2 ug/ml. Strong membranous and cytoplasmic HRP-DAB brown staining highlights immune cell populations within stromal and peri-glandular regions, consistent with detection of tumor-infiltrating lymphocytes and plasma cells within the tumor microenvironment. The staining pattern demonstrates immune cell distribution in malignant tissue, while epithelial tumor cells show variable staining. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free CD38 antibody (rCD38/6447) as confirmation of integrity and purity.

Negative control: IHC staining of FFPE human brain tissue using CD38 antibody (clone rCD38/6447) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.