CD38 Antibody / Microarray Specificity Validated Antibody [clone CD38/4328] (V9174)

CD38 Antibody human spleen and Raji lysate WB. Western blot analysis of CD38 / plasma cell marker expression in human spleen tissue and Raji cell lysate using CD38 antibody clone CD38/4328. Lane 1: human spleen lysate, Lane 2: human Raji cell lysate. A band is detected at approximately 40-45 kDa, consistent with the predicted molecular weight of CD38, with higher apparent molecular weight reflecting glycosylation of this cell surface protein and aligning with expression in immune cell-rich tissues and B cell-derived lines.

Western blot testing of human Raji cell lysate using CD38 antibody (clone CD38/4328). Expected molecular weight: 34-46 kDa depending on glycosylation level.

IHC staining of FFPE human tonsil tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

CD38 Antibody human prostate carcinoma tissue IHC. Immunohistochemistry analysis of CD38 / plasma cell marker expression in FFPE human prostate carcinoma tissue using CD38 antibody clone CD38/4328 at 2 ug/ml. Strong membranous and cytoplasmic HRP-DAB brown staining is observed in tumor-associated epithelial structures, with positive signal highlighting infiltrating immune cells within the tumor microenvironment. The staining pattern demonstrates prominent immune cell presence within malignant tissue, while surrounding stromal elements show lower background signal. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

Negative control: IHC staining of FFPE human cerebral cortex tissue with CD38 antibody (clone CD38/4328) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

CD38 Antibody microarray validation. Analysis of HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins using CD38 Antibody clone CD38/4328 demonstrates highly selective binding to CD38 as the top-ranked target. Z-score of 100.31 indicates strong signal intensity, while the S-score of 74.55 reflects a substantial separation from the next highest-ranking proteins, supporting high specificity. Z-score represents signal strength in standard deviations above the array mean, and S-score reflects the difference between consecutive ranked targets, indicating relative target specificity.