CD36 Antibody / Platelet glycoprotein 4 (FY13262)

Flow Cytometry analysis of human HEL cells using anti-CD36 antibody. Overlay histogram showing HEL cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD36 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of CD36 using anti-CD36 antibody. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD36 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected between an approximately 70 and 80 kDa in both samples, running well above the predicted ~53 kDa mass but consistent with the highly N-glycosylated mature form of the CD36 scavenger receptor reported in the literature, being observed at up to ~90 kDa.