CD35 Antibody / CR1 Complement Receptor 1 Antibody [clone CR1/6378] (V9752)

CD35 Antibody Tonsil IHC. Immunohistochemistry of CD35 / CR1 in FFPE human tonsil tissue using mouse monoclonal CD35 antibody, clone CR1/6378. HRP-DAB brown staining highlights a follicular dendritic cell network within germinal centers, showing a characteristic mesh-like pattern consistent with complement receptor localization, while surrounding lymphocytes show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CD35 Antibody Spleen IHC. Immunohistochemistry of CD35 / CR1 in FFPE human spleen tissue using mouse monoclonal CD35 antibody, clone CR1/6378. HRP-DAB brown staining highlights a follicular dendritic cell network within splenic white pulp, showing a reticular mesh-like pattern consistent with complement receptor localization in germinal center-associated stromal cells, while surrounding lymphoid cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free CD35 antibody (clone CR1/6378) as confirmation of integrity and purity.

CD35 Antibody Microarray Validation. Protein microarray analysis of CD35 / CR1 using mouse monoclonal CD35 antibody, clone CR1/6378, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to the target protein CD35 (CR1), with minimal reactivity toward non-target proteins. Signal intensity is strongly enriched for CR1 relative to all other proteins, supporting a high level of specificity. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, indicating relative target specificity. The observed signal profile confirms selective recognition of CD35 in a proteome-wide context.