CD34 Antibody for IHC / WB / Rodent Reactive Antibody (RQ6372)

CD34 Antibody Rat Brain IHC. Immunohistochemistry analysis of CD34 expression in FFPE rat brain demonstrates membranous HRP-DAB brown staining in vascular endothelial cells highlighting fine capillary networks and small vessel structures throughout neural tissue, while neuronal and glial cells remain largely negative. The staining pattern supports visualization of cerebral microvasculature and endothelial cell distribution in rat brain. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

CD34 Antibody Rat Kidney IHC. Immunohistochemistry analysis of CD34 expression in FFPE rat kidney demonstrates membranous HRP-DAB brown staining in vascular endothelial cells within glomerular capillary tufts and interstitial vessels, highlighting fine microvascular networks, while renal tubular epithelial cells remain largely negative. The staining pattern supports visualization of renal vascular architecture and endothelial cell localization in rat kidney. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

CD34 Antibody Rodent WB. Western blot analysis of CD34 expression in rodent tissue lysates. Lane 1: rat heart lysate, Lane 2: rat skeletal muscle lysate, Lane 3: rat lung lysate, Lane 4: mouse lung lysate. A band is detected at approximately 90-110 kDa, consistent with the predicted molecular weight of CD34 following extensive glycosylation. A lower band near the expected core molecular weight may represent less glycosylated or partially processed forms of the protein. The higher apparent molecular weight reflects known post-translational modification of CD34 as a heavily glycosylated cell surface protein, with stronger signal in lung tissues aligning with endothelial enrichment.