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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Cluster of Differentiation 34 (CD34) is a transmembrane sialomucin glycoprotein encoded by the CD34 gene and is widely expressed on vascular endothelial cells as well as hematopoietic stem and progenitor cells. It plays a key role in cell adhesion and migration within vascular and stem cell niches and is a defining marker of endothelial identity. CD34 Antibody for IF enables high-resolution visualization of endothelial cells in fluorescence-based imaging, where it reveals detailed vascular architecture and spatial organization within tissues and cultured systems.
CD34 antibody, also known as endothelial marker antibody or hematopoietic stem cell marker antibody, produces a characteristic membranous staining pattern in immunofluorescence that outlines the luminal surface of endothelial cells. This results in clear visualization of capillaries, branching microvessels, and continuous vascular networks, allowing researchers to map vessel architecture with high spatial precision. The fluorescent signal provides sharp contrast against surrounding stromal and epithelial compartments, making CD34 particularly effective for defining vascular boundaries in complex tissue environments.
This CD34 Antibody for IF is uniquely positioned for fluorescence-based imaging of vascular networks, where preservation of spatial relationships and cellular morphology is essential. Immunofluorescence allows direct visualization of endothelial cell arrangement, vessel branching patterns, and microvascular density within intact tissue sections or cell-based models. Compared to chromogenic detection methods, IF provides enhanced resolution and the ability to perform multicolor imaging, enabling simultaneous analysis of CD34 with additional cellular markers.
As a rabbit polyclonal antibody, this CD34 antibody recognizes multiple epitopes across the target protein, contributing to strong and uniform fluorescence signal across endothelial surfaces. This multi-epitope recognition enhances detection sensitivity and supports consistent labeling of vascular structures even in samples with variable antigen presentation or fixation conditions. The resulting signal is continuous and well-defined, supporting accurate visualization of vessel morphology.
In multicolor immunofluorescence applications, CD34 is frequently combined with markers such as CD31, VE-cadherin, or cytoskeletal proteins to examine endothelial cell organization and interactions within the vascular microenvironment. Co-staining approaches allow detailed analysis of vessel integrity, endothelial junctions, and surrounding cellular context, providing insight into angiogenesis, vascular remodeling, and tissue organization.
CD34 expression visualized by immunofluorescence may also be detected in progenitor cell populations and select stromal compartments depending on tissue type, but its most prominent and consistent localization remains within vascular endothelial cells. The distinct membranous fluorescence pattern enables clear identification of these cells even within densely structured tissues.
Overall, CD34 Antibody for IF provides strong, continuous membranous fluorescence signal and high-resolution visualization of vascular networks. Its ability to delineate endothelial structures with spatial precision makes it highly effective for studying angiogenesis, vascular organization, and endothelial cell biology in immunofluorescence-based imaging systems.
This antibody is part of our CD34 antibody collection, supporting research into stem cell biology, endothelial markers, and tumor angiogenesis.
The stated application concentrations are suggested starting amounts. Titration of the CD34 Antibody for IF / CD34 Immunofluorescence Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Human partial recombinant protein (AA 151-385) was used as the immunogen for this CD34 antibody.
After reconstitution, the CD34 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
CD34 immunofluorescence antibody, CD34 endothelial marker antibody, vascular endothelial IF antibody, CD34 fluorescence antibody, CD34 vessel marker antibody
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