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Home >> Antibodies >> CD34 Antibody

CD34 Antibody [clone QBEnd/10] (V2067)

  Catalog No Formulation Size Price (USD)  
Image V2067-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2067-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2067SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2067IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of human tonsil (10X) stained with CD34 antibody (QBEnd/10).
IHC testing of human tonsil (20X) stained with CD34 antibody (QBEnd/10).
Flow cytometry staining of human Jukat cells with CD34 antibody (clone QBEnd/10); Red=isotype control, Blue= CD34 antibody.
SDS-PAGE analysis of purified, BSA-free CD34 antibody (clone QBEnd/10) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, lambda
Clone Name QBEnd/10
Purity Protein G purified antibody
Buffer 1X PBS, pH 7.4
Gene ID 947
Localization Cell surface
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CD34 antibody is available for research use only.
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Description

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85% of angiosarcoma and Kaposi�s sarcoma, but with a lower specificity.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD34 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Storage

Store the CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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