• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> CD34 Antibody Cocktail

CD34 Antibody Cocktail [clone QBEnd/10 + HPCA1/763] (V2999)

  Catalog No Formulation Size Price (USD)  
Image V2999-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
Image
V2999-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
Image
V2999SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
Image
V2999IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD34 antibody (QBEnd/10 + HPCA1/763)
IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with CD34 antibody (QBEnd/10 + HPCA1/763)
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with CD34 antibody (QBEnd/10 + HPCA1/763)
IHC: Formalin-fixed, paraffin-embedded human placenta stained with CD34 antibody (QBEnd/10 + HPCA1/763)
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, lambda
Clone Name QBEnd/10 + HPCA1/763
Purity Protein G affinity chromatography
UniProt P28906
Localization Cell surface
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This CD34 antibody cocktail is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

Application Notes

Optimal dilution of the CD34 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10) and recombinant full-length human protein (HPCA1/763) were used as the immunogen for the CD34 antibody cocktail.

Storage

Store the CD34 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.