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Home >> Antibodies >> Recombinant CD31 Antibody / Rabbit Monoclonal

Recombinant CD31 Antibody / Rabbit Monoclonal [clone RMCD31-1] (V7214)

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  Catalog No Formulation Size Price (USD)  
Image V7214-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7214-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7214SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7214IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Western blot testing of human ThP1 cell lysate with recombinant CD31 antibody (clone RMCD31-1). Expected molecular weight: 83-130 kDa depending on level of glycosylation.
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Western blot testing of human ThP1 cell lysate with recombinant CD31 antibody (clone RMCD31-1). Expected molecular weight: 83-130 kDa depending on level of glycosylation.
IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone RMCD31-1). HIER: steam sections in 1mM EDTA, pH 9, for 10-20 min.
Flow cytometry testing of PFA-fixed human Jurkat cells with recombinant CD31 antibody (clone RMCD31-1); Red=isotype control, Blue= recombinant CD31 antibody.
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name RMCD31-1
Purity Protein A affinity
Gene ID 5175
Localization Cell surface and cytoplasmic
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant CD31 antibody is available for research use only.
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Description

CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

Application Notes

Titering of the recombinant CD31 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A full length recombinant human protein was used as the immunogen for the recombinant CD31 antibody.

Storage

Store the recombinant CD31 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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   2.   View all CD31 antibodies

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