CD31 Polyclonal Antibody / PECAM-1 Rabbit Polyclonal Antibody (R31053)

CD31 Polyclonal Antibody in Human Placenta by IF. Immunofluorescence staining of FFPE human placental sections using CD31 Polyclonal Antibody. CD31 immunoreactivity is shown in green with DAPI nuclear counterstaining in blue. The image demonstrates distinct staining associated with vascular structures throughout the placental tissue, consistent with the established endothelial distribution of PECAM-1. HIER was performed by steaming sections in pH 8 EDTA buffer for 20 min. The defined vascular staining demonstrates tissue localization using the PECAM-1 Rabbit Polyclonal Antibody.

CD31 Polyclonal Antibody in Human Liver Cancer by IF. Immunofluorescence staining of FFPE human liver cancer sections using CD31 Polyclonal Antibody. Red CD31 immunoreactivity highlights vascular structures distributed within the tumor tissue, while DAPI nuclear counterstaining is shown in blue. The staining pattern provides visualization of PECAM-1-positive vasculature within the malignant tissue. HIER was performed by steaming sections in pH 8 EDTA buffer for 20 min, providing fluorescence-based validation of this PECAM-1 Rabbit Polyclonal Antibody.

CD31 Polyclonal Antibody in Human Placenta by IHC. Immunohistochemistry of FFPE human placental sections using CD31 Polyclonal Antibody. Brown immunoreactivity distinctly outlines vascular endothelial structures within the placental tissue, producing strong contrast against the hematoxylin-counterstained background. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. The vascular staining pattern supports detection of PECAM-1 with the PECAM-1 Rabbit Polyclonal Antibody.

CD31 Polyclonal Antibody in Human Liver Cancer by IHC. Immunohistochemistry of FFPE human liver cancer sections using CD31 Polyclonal Antibody. Brown immunoreactivity highlights vascular structures within the malignant tissue, demonstrating localization of CD31-positive endothelium within the tumor microenvironment. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. This result complements the corresponding fluorescence staining and supports tissue-based detection with the PECAM-1 Rabbit Polyclonal Antibody.

CD31 Polyclonal Antibody in Human Liver Cancer Vasculature by IHC. Immunohistochemistry of FFPE human liver cancer sections using CD31 Polyclonal Antibody at 2 ug/ml. Distinct brown staining is concentrated along vascular endothelial structures within the tumor tissue, providing clear visualization of PECAM-1-positive vessels against the hematoxylin-counterstained background. Heat-mediated antigen retrieval was performed in pH 8 EDTA buffer, and sections were blocked with 10% goat serum before antibody incubation. This additional liver cancer field reinforces endothelial detection with the PECAM-1 Rabbit Polyclonal Antibody.

CD31 Polyclonal Antibody in Human Placental Vasculature by IHC. Immunohistochemistry of FFPE human placental sections using CD31 Polyclonal Antibody at 2 ug/ml. Strong brown immunoreactivity delineates endothelial cells associated with placental vascular structures, while surrounding tissue shows substantially less staining. Heat-mediated antigen retrieval was performed in pH 8 EDTA buffer, followed by blocking with 10% goat serum. The pronounced endothelial pattern provides additional validation of PECAM-1 localization using the PECAM-1 Rabbit Polyclonal Antibody.

CD31 Polyclonal Antibody in Six Human Cell Lines by WB. Western blot analysis using CD31 Polyclonal Antibody with 30 ug of reduced lysate per lane. Lane 1: Jurkat; lane 2: U937; lane 3: THP-1; lane 4: HEL; lane 5: HepG2; lane 6: PC-3. CD31 is a heavily glycosylated membrane protein with an expected apparent molecular weight broadly around 83-130 kDa depending on glycosylation state. The blot demonstrates differential PECAM-1 signal across the six human cell lines and provides biochemical validation for the PECAM-1 Rabbit Polyclonal Antibody.

CD31 Polyclonal Antibody in HUVEC and THP-1 Cells by WB. Western blot analysis using CD31 Polyclonal Antibody with 30 ug of reduced lysate per lane. Lanes 1 and 2 contain human HUVEC lysates, while lane 3 contains human THP-1 cell lysate. CD31/PECAM-1 is extensively glycosylated and commonly migrates within an approximately 83-130 kDa range rather than at a single molecular weight. Detection in endothelial HUVEC samples and THP-1 cells provides complementary western blot validation and supports use of the PECAM-1 Rabbit Polyclonal Antibody for detection of endogenous CD31.