Mouse CD31 Antibody / Rat PECAM-1 Antibody (RQ6065)

Mouse CD31 Antibody staining of rat cardiac vasculature by IHC.Immunohistochemistry of FFPE rat heart sections using the Mouse CD31 Antibody. Brown DAB staining is visible along vascular and endothelial structures distributed between cardiac muscle fibers, consistent with the vascular localization of CD31/PECAM1. Nuclei are counterstained blue. This Rat PECAM-1 Antibody was tested following heat-induced epitope retrieval by boiling sections in pH 8 EDTA for 20 minutes and allowing them to cool before antibody incubation.

Mouse CD31 Antibody staining of rat kidney vasculature by IHC.Immunohistochemistry of FFPE rat kidney sections using the Mouse CD31 Antibody. Distinct brown staining highlights vascular structures within the renal tissue, with prominent labeling surrounding vessel profiles and comparatively limited staining across much of the surrounding parenchyma. Nuclei are counterstained blue. The Rat PECAM-1 Antibody was tested following heat-induced epitope retrieval by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before antibody incubation.

Mouse CD31 Antibody staining of rat hepatic vessels by IHC.Immunohistochemistry of FFPE rat liver sections using the Mouse CD31 Antibody. Brown DAB signal is concentrated around prominent vascular structures, while the surrounding hepatic parenchyma shows substantially less staining. The distribution is consistent with detection of endothelial PECAM1 within the liver vasculature. Nuclei are counterstained blue. Testing of this Rat PECAM-1 Antibody used heat-induced epitope retrieval in pH 8 EDTA for 20 minutes followed by cooling.

Mouse CD31 Antibody staining of rat pulmonary vasculature by IHC. Immunohistochemistry of FFPE rat lung sections using the Mouse CD31 Antibody. Brown staining is visible along vascular and endothelial profiles within the pulmonary tissue, including structures adjacent to airways and alveolar regions. The staining pattern supports recognition of PECAM1-associated vascular endothelium. Nuclei are counterstained blue. This Rat PECAM-1 Antibody was tested after heat-induced epitope retrieval by boiling sections in pH 8 EDTA for 20 minutes and allowing them to cool.

Mouse CD31 Antibody staining of rat intestinal vasculature by IHC. Immunohistochemistry of FFPE rat colon sections using the Mouse CD31 Antibody. Brown DAB staining highlights vascular profiles within the mucosa and supporting tissue, producing a distribution consistent with endothelial CD31/PECAM1 expression. The epithelial architecture remains readily distinguishable with blue nuclear counterstaining. The Rat PECAM-1 Antibody was tested following heat-induced epitope retrieval by boiling sections in pH 8 EDTA for 20 minutes followed by cooling.

Mouse CD31 Antibody staining of mouse renal microvasculature by IHC. Immunohistochemistry of FFPE mouse kidney sections using the Mouse CD31 Antibody. Strong brown staining outlines vascular structures and capillary profiles within the renal tissue, including prominent labeling associated with glomerular and adjacent vascular compartments. Nuclei are counterstained blue. The observed pattern is consistent with PECAM1 expression in vascular endothelial cells. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before testing.

Mouse CD31 Antibody detection of mouse kidney endothelium by IHC. Immunohistochemistry of a second FFPE mouse kidney section using the Mouse CD31 Antibody. Brown DAB signal delineates endothelial structures within the renal parenchyma, including vascular and glomerular-associated profiles, against a predominantly blue nuclear counterstain. This additional field demonstrates PECAM1-associated vascular staining in mouse kidney. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes and allowing them to cool before antibody incubation.

Mouse CD31 Antibody staining of mouse colon vasculature by IHC. Immunohistochemistry of FFPE mouse colon sections using the Mouse CD31 Antibody. Brown staining is distributed along vascular profiles throughout the intestinal mucosa and supporting tissue, while the glandular architecture is clearly visualized by blue nuclear counterstaining. The pattern is consistent with endothelial PECAM1 localization within the colon. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA for 20 minutes followed by cooling before antibody incubation.

Mouse CD31 Antibody detection of PECAM1 in rat and mouse tissues by WB.Western blot testing of tissue lysates with the Mouse CD31 Antibody. Lane 1: rat lung; lane 2: rat spleen; lane 3: rat heart; lane 4: mouse lung. A prominent immunoreactive band is detected at approximately 100-110 kDa across the tested samples. CD31/PECAM1 has a predicted molecular weight range of approximately 83-130 kDa depending on its level of glycosylation, making the observed migration consistent with the mature glycoprotein. This Rat PECAM-1 Antibody therefore detects a band within the expected size range in rat tissues while also demonstrating recognition of mouse PECAM1 in mouse lung lysate.