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Home >> Antibodies >> CD31 Antibody / PECAM-1

CD31 Antibody / PECAM-1 [clone rPECAM1/6439] (V8788)

  Catalog No Formulation Size Price (USD)  
Image V8788-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V8788-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V8788SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with CD31 antibody (clone rPECAM1/6439). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD31 antibody (rPECAM1/6439) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rPECAM1/6439
Purity Protein A/G affinity
UniProt P16284
Localization Cell surface and cytoplasm of endothelial cells
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD31 antibody is available for research use only.
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Description

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Application Notes

Optimal dilution of the CD31 antibody should be determined by the researcher.

Immunogen

Full length recombinant human CD31 protein was used as the immunogen for the CD31 antibody.

Storage

Aliquot the CD31 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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