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Home >> Antibodies >> CD30 Antibody

CD30 Antibody [clone Ki-1/779] (V2992)

  Catalog No Formulation Size Price (USD)  
Image V2992-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2992-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2992SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2992IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (clone Ki-1/779).
SDS-PAGE Analysis of Purified, BSA-Free CD30 Antibody (clone Ki-1/779). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name Ki-1/779
Purity Protein G affinity chromatography
UniProt P28908
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This CD30 antibody is available for research use only.
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Description

CD30 is the receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. [UniProt]

Application Notes

Optimal dilution of the CD30 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the CD30 antibody.

Storage

Store the CD30 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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