CD3 epsilon Antibody for IF / CD3e Immunofluorescence Antibody (R31519)

Western blot testing of CD3 epsilon antibody and Lane 1: Jurkat; 2: CEM lysate. Expected/observed molecular weight ~23 kDa.

Western blot testing of CD3 epsilon antibody and recombinant human protein (0.5ng)

IHC-P: CD3 epsilon antibody testing of human tonsil tissue. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC-P: CD3 epsilon antibody testing of mouse spleen tissue. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC-P staining of rat spleen tissue with CD3 epsilon antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC-F staining of rat spleen tissue with CD3 epsilon antibody.

IHC-F staining of mouse spleen tissue with CD3 epsilon antibody.

CD3 epsilon Antibody for IF. Immunofluorescence analysis of CD3 Epsilon / CD3E antibody in FFPE human tonsil tissue using a rabbit polyclonal CD3e Immunofluorescence Antibody. CD3-positive T lymphocytes are visualized as bright pink membrane-associated and cytoplasmic fluorescence, predominantly localized within interfollicular regions, consistent with T-cell zone distribution. CD68 monoclonal antibody (green) highlights macrophages within the tissue, while DAPI (blue) marks cell nuclei. The staining demonstrates clear spatial separation between T-cell and macrophage populations and enables visualization of immune cell organization within lymphoid architecture. Heat-induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to staining.

CD3 epsilon Antibody for IF. Immunofluorescence analysis of CD3 Epsilon / CD3E antibody in FFPE human tonsil tissue using a rabbit polyclonal CD3e Immunofluorescence Antibody. CD3-positive T lymphocytes are detected as bright pink membrane-associated and cytoplasmic fluorescence predominantly within interfollicular and paracortical regions, while CD20 monoclonal antibody (green) labels B cells within follicular structures. DAPI (blue) marks cell nuclei, providing clear architectural context. The staining highlights the spatial segregation of T-cell and B-cell compartments within tonsillar lymphoid tissue. Heat-induced epitope retrieval was performed by steaming tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining.