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Home >> Antibodies >> CD3 epsilon Antibody

CD3 epsilon Antibody (R31519)

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  Catalog No Formulation Size Price (USD)  
Image R31519 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of CD3 epsilon antibody and Lane 1: Jurkat; 2: CEM lysate. Expected/observed molecular weight ~23 kDa.
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Western blot testing of CD3 epsilon antibody and Lane 1: Jurkat; 2: CEM lysate. Expected/observed molecular weight ~23 kDa.
Western blot testing of CD3 epsilon antibody and recombinant human protein (0.5ng)
IHC-P: CD3 epsilon antibody testing of human tonsil tissue. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC-P: CD3 epsilon antibody testing of mouse spleen tissue. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC-P staining of rat spleen tissue with CD3 epsilon antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC-F staining of rat spleen tissue with CD3 epsilon antibody.
IHC-F staining of mouse spleen tissue with CD3 epsilon antibody.
Immunofluorescent staining of FFPE human tonsil tissue with CD3 epsilon antibody (pink) and CD68 mAb (green) at 1ug/ml, and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human tonsil tissue with CD3 epsilon antibody (pink) and CD20 mAb (green) at 2ug/ml, and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 916
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
IHC (Frozen) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Immunofluorescence : 1-2ug/ml
Limitations This CD3 epsilon antibody is available for research use only.
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Description

CD3 epsilon is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. Together with CD3gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, CD3e forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the e, g and d polypeptides are located in the same cluster on chromosome 11. The e-polypeptide plays an essential role in T-cell development.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the CD3 epsilon antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 23-207) was used as the immunogen for this CD3 epsilon antibody.

Storage

After reconstitution, the CD3 epsilon antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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