- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD3 epsilon Antibody [clone CDLA3e-1] (V3761)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V3761-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
|
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V3761-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V3761SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | CDLA3e-1 |
| Purity | Protein G affinity chromatography |
| UniProt | P07766 |
| Localization | Cell surface and cytoplasmic |
| Applications | Flow cytometry : 1-2ug/10^6 cells Western blot : 1-2ug/ml |
| Limitations | This CD3 epsilon antibody is available for research use only. |
|
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This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
The stated application concentrations are suggested starting points. Titration of the CD3 epsilon antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 23-119 were used as the immunogen for the CD3 epsilon antibody.
Store the CD3 epsilon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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