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Home >> Antibodies >> CD2AP Antibody

CD2AP Antibody (RQ4204)

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Image RQ4204 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) COLO320, 5) 22RV1 6) SK-OV-3, 7) rat stomach, 8) rat liver and 9) mouse liver lysate wtih CD2AP antibody at 0.5ug/ml. Predicted molecular weight: 71-80 kDa.
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Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) COLO320, 5) 22RV1 6) SK-OV-3, 7) rat stomach, 8) rat liver and 9) mouse liver lysate wtih CD2AP antibody at 0.5ug/ml. Predicted molecular weight: 71-80 kDa.
Western blot testing of human 1) Jurkat, 2) A431, 3) HeLa and 4) K562 cell lysate wtih CD2AP antibody. Predicted molecular weight: 71-80 kDa.
Immunoprecipitation of CD2AP protein from 500ug of human Jurkat whole cell lysate with 2ug of CD2AP antibody.
Flow cytometry testing of fixed and permeabilized human K562 cells with CD2AP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.
IHC staining of FFPE mouse kidney tissue with CD2AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with CD2AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with CD2AP antibody (green) at 5ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human K562 cells with CD2AP antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9Y5K6
Applications Western Blot : 1-2ug/ml
Flow Cytometry : 1-3ug/million cells
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
ELISA : 0.1-0.5ug/ml
Immunoprecipitation : 2ug antibody/500ug lysate
Limitations This CD2AP antibody is available for research use only.
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    Reactivity : Human

Description

CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

Application Notes

Optimal dilution of the CD2AP antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids K253-K337 was used as the immunogen for the CD2AP antibody.

Storage

After reconstitution, the CD2AP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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