CD2AP Antibody / Cytoskeletal Adapter Protein Antibody (RQ4204)

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody Human, Mouse and Rat WB. Western blot analysis of 1) human HeLa, 2) human 293T, 3) human MCF7, 4) human COLO320, 5) human 22RV1, 6) human SK-OV-3, 7) rat stomach, 8) rat liver, and 9) mouse liver lysates using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody. A specific immunoreactive band is detected at approximately 71-80 kDa across the tested human, rat, and mouse samples, consistent with the predicted molecular weight range of CD2AP. These results demonstrate reliable cross-species detection of CD2AP, a multifunctional adaptor protein involved in cytoskeletal organization, receptor endocytosis, and intracellular signaling.

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody Human Cell Line WB. Western blot analysis of 1) human Jurkat, 2) human A431, 3) human HeLa, and 4) human K562 cell lysates using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody. A specific immunoreactive band is detected at approximately 71-80 kDa in all four cell lines, consistent with the predicted molecular weight range of CD2AP. These results demonstrate reliable detection of CD2AP, a multifunctional adaptor protein that links membrane receptors to the actin cytoskeleton and regulates cell adhesion, receptor endocytosis, and intracellular signaling.

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody Immunoprecipitation Validation. Immunoprecipitation of CD2AP from human Jurkat whole cell lysate using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody, followed by western blot detection. Lane 1: Input Jurkat whole cell lysate. Lane 2: Control IgG immunoprecipitation (negative control). Lane 3: CD2AP antibody immunoprecipitation. A specific band corresponding to CD2AP is detected at approximately 71-80 kDa in the input and immunoprecipitated sample, while no corresponding CD2AP band is observed in the control IgG lane. The IgG heavy chain is visible at approximately 50 kDa, confirming successful immunoprecipitation.

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody Human K562 Flow Cytometry. Flow cytometric analysis of fixed and permeabilized human K562 cells using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody at 1 ug/10^6 cells. Cells were blocked with goat serum prior to staining. Red: unstained cells. Green: isotype control. Blue: CD2AP antibody. The distinct rightward shift of the CD2AP antibody signal relative to the isotype control demonstrates specific intracellular detection of CD2AP in K562 cells.

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody Mouse Kidney IHC. Immunohistochemical staining of FFPE mouse kidney tissue using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody. CD2AP expression is detected predominantly in glomerular podocytes and renal tubular epithelial cells, consistent with its established role in maintaining the slit diaphragm, regulating actin cytoskeletal organization, and supporting glomerular filtration barrier integrity. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Immunoreactivity was visualized using an HRP/DAB detection system.

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody Rat Kidney IHC. Immunohistochemical staining of FFPE rat kidney tissue using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody. CD2AP expression is detected predominantly in glomerular podocytes and renal tubular epithelial cells, consistent with its essential role in maintaining slit diaphragm architecture, regulating actin cytoskeletal dynamics, and preserving glomerular filtration barrier function. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Immunoreactivity was visualized using an HRP/DAB detection system.

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody HeLa Immunofluorescence. Immunofluorescent staining of FFPE human HeLa cells using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody (green) with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6.0 citrate buffer for 20 minutes prior to staining. Cells were incubated with the primary antibody at 5 ug/ml. CD2AP displays predominantly cytoplasmic staining, consistent with its role as a cytoskeletal adaptor protein that links membrane-associated signaling complexes to the actin cytoskeleton and regulates receptor trafficking and cell adhesion.

CD2AP Antibody / Cytoskeletal Adapter Protein Antibody K562 Immunofluorescence. Immunofluorescent staining of FFPE human K562 cells using CD2AP Antibody / Cytoskeletal Adapter Protein Antibody (red) with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6.0 citrate buffer for 20 minutes prior to staining. Cells were incubated with the primary antibody at 2 ug/ml. CD2AP displays predominantly cytoplasmic staining, consistent with its function as a cytoskeletal adaptor protein involved in actin organization, receptor endocytosis, and intracellular signaling.