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Home >> Antibodies >> CD28 Antibody

CD28 Antibody (F49999)

  Catalog No Formulation Size Price (USD)  
Image F49999-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49999-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Confocal immunofluorescent analysis of CD28 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Western blot analysis of CD28 antibody and MDA-MB231 lysate. Expected molecular weight depending on level of glycosylation: ~25-44 kDa (monomer), ~50-90kDa (dimer).
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Rabbit
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P10747
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Limitations This CD28 antibody is available for research use only.
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Description

CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.

Application Notes

Titration of the CD28 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 182-208 from the human protein was used as the immunogen for this CD28 antibody.

Storage

Aliquot the CD28 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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