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Home >> Antibodies >> CD28 Antibody PE Conjugate

CD28 Antibody PE Conjugate [clone CB28] (V2051PE)

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  Catalog No Formulation Size Price (USD)  
Image V2051PE-100T 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 Tests 519
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Availability 1-3 business days
Species Reactivity Human. Other species not known.
Format PE Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CB28
Purity Protein G affinity chromatography
Gene ID 940 (Human)
Localization Cell surface
Applications Flow Cytometry : 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood
Immunofluorescence : 1:50-1:100
Limitations This CD28 antibody is available for research use only.
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Description

This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Immunogen

Recombinant human CD28 protein was used as the immunogen for this antibody.

Storage

Store the CD28 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

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