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Home >> Antibodies >> CD28 Antibody

CD28 Antibody [clone C28/1636] (V3258)

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  Catalog No Formulation Size Price (USD)  
Image V3258-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3258-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3258SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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SDS-PAGE Analysis of Purified, BSA-Free CD28 Antibody (clone C28/1636). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free CD28 Antibody (clone C28/1636). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name C28/1636
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P10747
Gene ID 940
Localization Cell surface
Applications Flow cytometry : 1-2ug/10^6 cells
Limitations This CD28 antibody is available for research use only.
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Description

CD28 is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Immunogen

Recombinant human protein was used as the immunogen for this CD28 antibody.

Storage

Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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