CD27 Antibody for WB / Cross-Species CD27 Western Blot Antibody [clone r137B4] (V6038)

CD27 Antibody for WB. Western blot analysis of CD27 / TNFRSF7 expression across species using Cross-Species CD27 Western Blot Antibody clone r137B4. Lane 1: human spleen lysate, Lane 2: mouse spleen lysate, Lane 3: rat spleen lysate, Lane 4: hamster spleen lysate, Lane 5: guinea pig spleen lysate, Lane 6: rabbit spleen lysate. A band is detected at approximately 55-60 kDa, consistent with glycosylated CD27, noting a lower predicted molecular weight for the core protein. CD27 is a glycosylated receptor, and this higher apparent molecular weight reflects N-linked glycosylation commonly observed for TNFRSF7 in immune tissues. The consistent banding pattern across species supports recognition of conserved CD27 epitopes and cross-species reactivity of clone r137B4.

CD27 Antibody for WB. Western blot analysis of CD27 / TNFRSF7 expression in human samples using Cross-Species CD27 Western Blot Antibody clone r137B4. Lane 1: human spleen lysate, Lane 2: Ramos B-cell lysate. A band is detected at approximately 55-60 kDa, consistent with glycosylated CD27, noting a lower predicted molecular weight for the core protein. CD27 is a glycosylated receptor, and this higher apparent molecular weight reflects N-linked glycosylation commonly observed in immune cells. The comparable banding pattern in spleen tissue and Ramos cells supports specific detection of CD27 and consistent performance of clone r137B4 across tissue and cell line samples.

Immunohistochemistry analysis of CD27 Molecule Antibody in FFPE human tonsil tissue. Membranous and cytoplasmic HRP-DAB brown staining is observed in scattered lymphocytes within interfollicular regions and germinal center areas, consistent with CD27 expression on activated and memory B and T lymphocyte populations, while surrounding negative lymphocytes remain unstained. Clone r137B4 demonstrates distinct cell surface localization. The inset shows PBS used in place of the primary antibody as a secondary-only negative control. Heat-induced epitope retrieval was performed in 10mM Tris with 1mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes.

SDS-PAGE Analysis of Purified CD27/CD27 molecule antibody (clone r137B4). Confirmation of Purity and Integrity of Antibody.