CD27 Antibody for FACS / Cross-Species CD27 Flow Cytometry Antibody (RQ6837)

CD27 Antibody Rat Spleen IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE rat spleen tissue using CD27 Immunohistochemistry Antibody. Strong membranous and cytoplasmic HRP-DAB brown staining is observed in lymphocyte populations within splenic white pulp regions, consistent with CD27 expression on T cells and memory B cells, while surrounding non-lymphoid areas show minimal staining. The staining highlights lymphoid architecture and immune cell localization in spleen tissue. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

CD27 Antibody Rat Lymph Node IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE rat lymph node tissue using CD27 Immunohistochemistry Antibody. Distinct membranous and cytoplasmic HRP-DAB brown staining is observed in lymphocyte populations within lymph node follicles and paracortical regions, consistent with CD27 expression on activated and memory T and B cells, while surrounding stromal elements remain largely negative. The staining pattern highlights lymphoid organization and immune cell compartmentalization within the node. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

CD27 Antibody Mouse Lymph Node IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE mouse lymph node tissue using CD27 Immunohistochemistry Antibody. Membranous and cytoplasmic HRP-DAB brown staining is observed in scattered lymphocyte populations within lymph node parenchyma, consistent with CD27 expression on activated and memory T and B cells, while adjacent non-lymphoid structures remain largely unstained. The distribution of positive cells supports identification of immune cell subsets within the lymph node microenvironment. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

CD27 Antibody for FACS. Flow cytometry analysis of CD27 / TNFRSF7 expression in mouse peripheral blood mononuclear cells using Cross-Species CD27 Flow Cytometry Antibody. Cells stained with CD27 antibody (blue) show a clear right-shifted population compared to isotype control (green) and unstained cells (red), indicating cell surface expression of CD27 on lymphocyte populations. The distinct separation supports accurate gating and identification of CD27-positive cells and highlights its utility for PBMC-based immune profiling in flow cytometry.