CD27 Antibody / Lymphoid Tissue Architecture Marker Antibody (R32373)

CD27 Antibody Human Spleen IHC. Immunohistochemical staining of FFPE human spleen tissue using CD27 Antibody / Lymphoid Tissue Architecture Marker Antibody following heat-induced epitope retrieval in pH8 EDTA buffer. The tissue was incubated with the primary antibody at 2 ug/ml, followed by an HRP-conjugated goat anti-rabbit secondary antibody and DAB chromogen for visualization. Distinct membranous and cytoplasmic staining is observed in numerous lymphoid cells within the splenic white pulp, consistent with the expected expression pattern of CD27 on activated T cells, memory B cells, and other lymphocyte subsets. CD27 Antibody / Lymphoid Tissue Architecture Marker Antibody is well suited for evaluating CD27 expression and lymphoid tissue organization in immunohistochemical studies.

IHC testing of FFPE human tonsil with CD27 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

CD27 Antibody Human and Mouse Lymphoid Cell WB. Western blot analysis of human Raji (lane 1), human Ramos (lane 2), human Daudi (lane 3), and mouse EL-4 (lane 4) whole cell lysates using CD27 Antibody / Lymphoid Tissue Architecture Marker Antibody. A primary antibody concentration of 0.5 ug/ml was used. A distinct band is detected at approximately 55-60 kDa in all four lymphoid cell lines. Although the predicted molecular weight of CD27 is approximately 29 kDa, the higher apparent molecular weight is consistent with extensive glycosylation of this cell surface receptor, which is known to migrate well above its calculated mass during SDS-PAGE. CD27 Antibody / Lymphoid Tissue Architecture Marker Antibody is suitable for detecting CD27 expression in human and mouse lymphoid cells by Western blot.