CD27 Antibody / Lymphoid Tissue Architecture Marker Antibody (R32373)

CD27 Antibody for WB. Western blot analysis of CD27 / TNFRSF7 expression in human HeLa cell lysate using CD27 Antibody. Lane 1: HeLa cell lysate. A band is detected at approximately 45–50 kDa, consistent with glycosylated CD27, noting a lower predicted molecular weight of approximately 29 kDa for the core protein. CD27 is a glycosylated receptor, and the higher apparent molecular weight reflects N-linked glycosylation that can influence migration on SDS-PAGE. The observed signal may reflect low-level or context-dependent expression and supports evaluation of CD27 distribution in studies of lymphoid tissue architecture.

IHC testing of FFPE human tonsil with CD27 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

CD27 Antibody for FACS. Flow cytometry analysis of CD27 / TNFRSF7 expression in human A549 cells using CD27 Antibody. Cells stained with CD27 antibody (blue) show a right-shifted population compared to isotype control (green) and unstained cells (red), indicating detectable CD27 signal under these conditions. The distribution supports evaluation of CD27 expression and background separation in non-lymphoid cell contexts and complements its use as a lymphoid tissue architecture marker for assessing immune cell localization in flow cytometry studies.

CD27 Antibody for IF. Immunofluorescence analysis of CD27 / TNFRSF7 expression in FFPE human K562 cells using CD27 Antibody (red) and DAPI (blue). Signal is observed at the cell periphery and cytoplasmic regions, consistent with CD27 localization in membrane-associated compartments, while nuclei are counterstained with DAPI (blue). The staining pattern supports evaluation of CD27 distribution in cellular contexts and complements its use as a lymphoid tissue architecture marker for assessing immune cell organization and localization. Heat-induced epitope retrieval was performed in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to antibody incubation.