CD27 Antibody / Immune Checkpoint Pathway Antibody [clone LPFS2/8838R] (V4224)

CD27 Antibody for Human LN IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human lymph node tissue using CD27 Antibody / Immune Checkpoint Pathway Antibody clone LPFS2/8838R. Prominent membranous HRP-DAB brown staining is observed in lymphocyte populations within follicular and paracortical regions, consistent with CD27 expression on activated and regulatory T and B cell subsets involved in immune signaling, while surrounding stromal elements remain largely negative. The staining pattern highlights immune regulatory compartments and supports evaluation of checkpoint-related signaling within lymphoid tissue architecture. Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

CD27 Antibody for Lymph Tissue IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human lymph node tissue using CD27 Antibody / Immune Checkpoint Pathway Antibody clone LPFS2/8838R. Strong membranous HRP-DAB brown staining is observed in lymphocyte populations within follicular and paracortical regions, consistent with CD27 expression on activated and regulatory T and B cell subsets involved in immune signaling, while surrounding stromal elements remain largely negative. The staining pattern highlights immune regulatory compartments and supports evaluation of checkpoint-related signaling within lymphoid tissue architecture. Inset: PBS used in place of primary Ab (secondary Ab negative control). Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free CD27 antibody (clone LPFS2/8838R) as confirmation of integrity and purity.